primary antibodies  (Proteintech)

Journal: Journal of Inflammation Research

Article Title: Catalpol Alleviates HFpEF via Inhibition of the S100A8-RAGE-NOX4 Inflammatory Axis in Murine Hearts

doi: 10.2147/JIR.S552741

Figure Lengend Snippet: Visualization of molecular docking results using interaction diagrams. ( A ) Molecular docking of CAT and ERK1. ( B ) Molecular docking of CAT and MMP2. ( C ) Molecular docking of CAT and NOX4. ( D ) Molecular docking of CAT and RAGE. ( E ) Molecular docking of CAT and S100A8.

Article Snippet: Primary antibodies against the following targets were used: S100A8 (15792-1-AP), RAGE (16346-1-AP), NOX4 (14347-1-AP), MMP2 (10373-2-AP), and GAPDH (60004-1-AP) from Proteintech (Wuhan, China); ERK1/2 (9102), phospho-ERK1/2 (p-ERK1/2, 4370), p65 (8242), and phospho-p65 (p-p65, 3033) from Cell Signaling Technology (Danvers, MA, USA).

Techniques:

Journal: Journal of Inflammation Research

Article Title: Catalpol Alleviates HFpEF via Inhibition of the S100A8-RAGE-NOX4 Inflammatory Axis in Murine Hearts

doi: 10.2147/JIR.S552741

Figure Lengend Snippet: CAT downregulated the S100A8-RAGE-NOX4 signaling pathway in HFpEF. ( A ) Representative WB bands of S100A8, RAGE, NOX4, ERK, p-ERK, P65, p-P65, and MMP2. ( B – G ) Quantification of protein expression levels analyzed using ImageJ software (n=3). ( H ) Quantitative analysis of IL-1β expression (n=3). ( I ) Quantitative analysis of IL-6 expression (n=3). ( J ) Quantitative analysis of TNF-α expression (n=3). Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Primary antibodies against the following targets were used: S100A8 (15792-1-AP), RAGE (16346-1-AP), NOX4 (14347-1-AP), MMP2 (10373-2-AP), and GAPDH (60004-1-AP) from Proteintech (Wuhan, China); ERK1/2 (9102), phospho-ERK1/2 (p-ERK1/2, 4370), p65 (8242), and phospho-p65 (p-p65, 3033) from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing, Software